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Then, 50 μL of the resulting mixture was incubated at 22°C-24°C for 5 min, and the absorbance was measured at regular intervals of 5 min for up to 1 h at 450 nm

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Mechanistically, this inhibition of glycolytic activity was attributable to destabilization of HIF-1α protein, possibly through α-KG-dependent PHD enzyme-mediated hydroxylation and enhanced degradation of HIF-1α protein (33)