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Importantly, vascular senescent cells are identified by key characteristics and biomarkers including cell cycle regulators (i.e., cdkn1a - p21, Cdkn2a - p16 and p19), altered lysosomal activity (senescence-associated β-galactosidase [SA-β-Gal]), DNA damage markers (53BP1 and γ-H2AX), telomere length and telomerase activity, senescent cell anti-apoptotic pathways (SCAPs), and SASP profile (IL-6, IL-1β, and tumor necrosis factor [TNF-], among many others) [31,45] [Figure 1]