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The proteome concentration of lysates was normalized to 2 mg/mL, and 100 μL was transferred to a LoBind Eppendorf tube containing 48 mg of urea

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1000mcg of methylcobalamin preservative free (concentrated) put into an acupuncture point by Laura 2500mcg of methylcobalamin given as given by one of our nurses with an intramuscular (IM) shot