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Considering extensive previous studies reporting that PVN neuronal activity is closely associated with gastrointestinal function 27,28 , we performed immunofluorescence staining to identify the neuronal types in the PVN marked by EGFP: ~ 92% of EGFP + cells were co-stained with a glutamate-specific antibody, while only 12% of EGFP + cells were co-localized with a GABA-specific antibody (Fig